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Alexander A Sousa
Alexander A Sousa
PhD Candidate, Harvard Medical School
Verified email at g.harvard.edu
Title
Cited by
Cited by
Year
Search-and-replace genome editing without double-strand breaks or donor DNA
AV Anzalone, PB Randolph, JR Davis, AA Sousa, LW Koblan, JM Levy, ...
Nature 576 (7785), 149-157, 2019
34042019
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
JS Chen, YS Dagdas, BP Kleinstiver, MM Welch, AA Sousa, ...
Nature 550 (7676), 407-410, 2017
11212017
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
BP Kleinstiver, AA Sousa, RT Walton, YE Tak, JY Hsu, K Clement, ...
Nature biotechnology 37 (3), 276-282, 2019
5292019
Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications
AE Friedland, R Baral, P Singhal, K Loveluck, S Shen, M Sanchez, ...
Genome biology 16, 1-10, 2015
3402015
High levels of AAV vector integration into CRISPR-induced DNA breaks
KS Hanlon, BP Kleinstiver, SP Garcia, MP Zaborowski, A Volak, SE Spirig, ...
Nature communications 10 (1), 4439, 2019
3142019
Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs
J Listgarten, M Weinstein, BP Kleinstiver, AA Sousa, JK Joung, J Crawford, ...
Nature biomedical engineering 2 (1), 38-47, 2018
2712018
Discovery of widespread type I and type V CRISPR-Cas inhibitors
ND Marino, JY Zhang, AL Borges, AA Sousa, LM Leon, BJ Rauch, ...
Science 362 (6411), 240-242, 2018
2332018
Designing and executing prime editing experiments in mammalian cells
JL Doman, AA Sousa, PB Randolph, PJ Chen, DR Liu
Nature protocols 17 (11), 2431-2468, 2022
452022
Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice
KA Everette, GA Newby, RM Levine, K Mayberry, Y Jang, T Mayuranathan, ...
Nature biomedical engineering 7 (5), 616-628, 2023
402023
Adenine transversion editors enable precise, efficient A• T-to-C• G base editing in mammalian cells and embryos
L Chen, M Hong, C Luan, H Gao, G Ru, X Guo, D Zhang, S Zhang, C Li, ...
Nature Biotechnology, 1-13, 2023
382023
Variants of Cpf1 (Cas12a) with altered PAM specificity
JK Joung, B Kleinstiver, A Sousa
US Patent 11,591,589, 2023
382023
Enhanced Proofreading Governs CRISPR-Cas9 Targeting Accuracy
JS Chen, YS Dagdas, BP Kleinstiver, MM Welch, AA Sousa, ...
Biophysical Journal 114 (3), 194a, 2018
82018
Variants of cpf1 (cas12a) with altered pam specificity
JK Joung, B KLEINSTIVER, A SOUSA
42018
Publisher Correction: Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
BP Kleinstiver, AA Sousa, RT Walton, YE Tak, JY Hsu, K Clement, ...
Nature biotechnology 38 (7), 901, 2020
32020
Author Correction: Adenine transversion editors enable precise, efficient A• T-to-C• G base editing in mammalian cells and embryos
L Chen, M Hong, C Luan, H Gao, G Ru, X Guo, D Zhang, S Zhang, C Li, ...
Nature biotechnology, 2024
2024
Development of a Prime Editing Strategy to Treat Mutations in the Crumbs Homologue 1 (CRB1) Gene
BL Costa, AA Sousa, YT Tsai, Y Li, IH Maumenee, DR Liu, SH Tsang, ...
Investigative Ophthalmology & Visual Science 64 (8), 3862-3862, 2023
2023
Development of a Prime Editing Strategy to Treat Mutations in the Crumbs Homologue 1 (CRB1) Gene
BL Da Costa, AA Sousa, YT Tsai, Y Li, IH Maumenee, DR Liu, SH Tsang, ...
MOLECULAR THERAPY 30 (4), 260-261, 2022
2022
Variants of CPF1 (CAS12a) With Altered PAM Specificity
JK Joung, B Kleinstiver, A Sousa
US Patent App. 17/244,738, 2021
2021
Enhancing Genome Editing with Engineered CRISPR Nucleases
RT Walton, AA Sousa, MM Welch, YE Tak, JY Hsu, K Clement, JE Horng, ...
MOLECULAR THERAPY 27 (4), 104-105, 2019
2019
AAV-Vector Integration into CRISPR-Induced Double-Stranded Breaks
B Gyorgy, BP Kleinstiver, SP Garcia, M Zaborowski, AA Sousa, SQ Tsai, ...
Molecular Therapy 26 (5), 432-433, 2018
2018
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